How Is Relative Retention Time Calculated?｜質量分析計・LC-MS/MSシステムのSCIEX

For research use only. Not for use in diagnostic procedures.

Answer

For analytes using an internal standard, the actual retention time (RT) of the internal standard peak (for the corresponding sample) is first determined. The expected RT for the analyte is determined by multiplying the specified expected RT by the ratio of the actual to the expected RT for the internal standard.