How Is the Peptide Score Calculated in BPV Flex™ Software 2.1?


日付: 03/31/2021
カテゴリー: Academia Omics , Pharma CRO , BioPharmaView Software

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For research use only. Not for use in diagnostic procedures.


Answer

MS/MS scoring provides a way to measure of the confidence of a matched theoretical peptide sequence to the experimental data. During matching, all theoretical peptides within the m/z tolerance are evaluated and scored if an MS/MS spectrum is available. The score indicates the probability that the peaks in a given MS/MS spectrum will match those of the theoretical peptide fragments by random chance. Therefore, the BPV Flex™ Software score calculation is derived from observed MS/MS fragments and the corresponding intensities, as well as the number of theoretical MS/MS fragments. A higher score indicates a more confident match and is reported as –Log10 (probability).