How Does the Score in the Peptide Sequencing Section of BioPharmaView™ Software 2.1 Work?


日付: 12/01/2017
カテゴリー: Academia Omics , Pharma CRO , BioPharmaView Software

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For research use only. Not for use in diagnostic procedures.


Answer

There are two components that must be considered to create the final score for each peptide in the peptide sequencing section of BioPharmaView™ software 2.1:
1) The precursor ion mass accuracy match (MS level)
2) the number of matches of the MS/MS fragment ions and their mass accuracy (MS 2 level). 

Posted: Feb 19, 2021

How is the score generated? How much weight is given to either criterium ? The software suggest a minimum value of 3 for proper identification, how is that values arrived at? thanks