Primer Purity Requirements for Sequencing and Fragment Analysis Using the GenomeLab™ GeXP Genetic Analysis System


日付: 11/30/2018
カテゴリー: GenomeLab GeXP Genetic Analysis System

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For research use only. Not for use in diagnostic procedures.


Answer

When ordering nucleic acid primers for use in either DNA sequencing or fragment analysis using the GenomeLab™ GeXP Genetic Analysis System or older CEQ genetic analyzers, be sure to select primers thatat the minimum have been purified via solid phase extraction. De-salted primers will not work well for sequencing, but they can be used for fragment analysis. Further purification techniques, such as reversed-phase or anion-exchange HPLC or even higher PAGE gel purification, can also be used but are only necessary if the primer is highly specific to its binding site on the template.