Creating an Ion Library for DIA-NN Software Using the Ion Library Generator in OneOmics™ Suite 3.3
| 日付: | 02/10/2023 |
| カテゴリー: | Academia Omics , Software |
For research use only. Not for use in diagnostic procedures.
Answer
The steps for creating an ion library for the DIA-NN software using the Ion Library Generator in the OneOmics™ Suite 3.3 software are described here. The DIA-NN software requires that the DDA data submitted for processing is uploaded in a specific text file format.
Navigate to the Launch page in the OneOmics suite, and then follow these steps to create a library:
1) Create or re-use a protein ID experiment. Select the Experiments tile and navigate to the uploaded DDA wiff files. CloudConnect software 1.8 can be used to upload the data set.
2) Select the Launch Processing button and then launch the Ion Library Generator. Then create a file name, choose a location for the results file. By default, a false discovery rate (FDR) value of 1% is chosen for proteins and peptides. Users can select two search options:
b) Multi-File ProteinPilot Search: This option allows for a classic ProteinPilot app search, where all of the files from the ProteinPilot app experiment are submitted as a single search, thereby generating a single group file. This type of search operates best when the IDA files are on the same retention time scales and protein grouping is a priority. Choose any additional settings and then execute data processing.
3) After the processing is done, navigate to the output files under the information symbol circled in orange in the image below.
4) Download the ion library for the DIA-NN software under the Data Store menu represented by the three dots.
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